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PAVED- A Software suite for  the analysis of epigenome-derived next generation sequencing data

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ANALYTICAL PIPELINE

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SYSTEM REQUIREMENTS

PAVED Package  

Example Data

Find Annotations

The utility findAnnotations finds annotations of the regions specified in .bed file from the .gff file.

Prerequisites

1) A .bed file and a .gff file

How to run it?

Type java -jar PAVED.jar findAnnotations -h to see list of parameters

findAnnotations utility takes as input two parameters 1) .bed file and 2).gff file and outputs the annotations for the regions specified in .bed file from the .gff file

Run the utility as follows:

java -jar C:\Britta\manuscript\Analysis\PAVED.jar findAnnotations -i C:\Britta\manuscript\Analysis\data\FAIRE\normalizedData\FaireRepvsInput.peaks -j C:\Britta\manuscript\Analysis\data\annotations\LmajorFriedlin_TriTrypDB-4.0.gff -o C:\Britta\manuscript\Analysis\data\FAIRE\normalizedData\FaireRepvsInput.annot


Here, "
C:\Britta\manuscript\Analysis\" is the location where the jar file is present on the local disk, -i is the peaks or valleys file in .bed format generated using paved, -j is the .gff file containing annotations and -o is the output file with annotations of regions in the .bed file if any.

Sample output

The sample output is as follows:
annotation output
The columns in the output file are as follows:

1) Chromosome name
2), 3) Start and end positions of a region as specified in the .bed file
4), 5) Start and end positions of the annotated region from the .gff file
6) Description of this region as specified in column 9 of the .gff file