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PAVED- A Software suite for  the analysis of epigenome-derived next generation sequencing data

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PAVED Package  

Example Data

Find Peaks

The utility findPeaks finds peaks from the fold change file generated using  foldChangeReadDepth to find peaks with values less than a certain number and certain region size.

Prerequisites

1) Find the fold change using the utility  foldChangeReadDepth.

How to run it?

Type java -jar PAVED.jar findPeaks -h to see list of parameters

findPeaks utility takes as input three parameters 1) fold change file in wiggle format, 2) minimum threshold value and 3) minimum region size value

Run the utility as follows:

java -jar C:\Britta\manuscript\Analysis\PAVED.jar findPeaks -i C:\Britta\manuscript\Analysis\data\FAIRE\normalizedData\FaireRepvsInput.wig -o C:\Britta\manuscript\Analysis\data\FAIRE\normalizedData\FaireRepvsInput.peaks -m 5 -n 10


Here, "
C:\Britta\manuscript\Analysis\" is the location where the jar file is present on the local disk, -i is the fold change wiggle file, -m is the parameter specifying minimum peak value, -n is the minimum region width and -o is the output file with regions that are above the thresholds presented.

Sample output

The sample output is as follows:
This shows for example that there is a peak between 350494 and 350509 on the chromosome LmjF05
peaks